Morfologia e anatomia foliar de Annona paludosa Aubl. (Annonaceae) que ocorre na ilha de Algodoal-PA

Abstract

In northeastern Pará, in the municipality of Maracanã, is located the restinga of Algodoal and Maiandeua. In this area two species of the Annonaceae family, Annona paludosa Aubl. and Annona glabra L., have been registered. From these species we selected A. paludosa Aubl. with the objective of studying its leaf anatomy. In order to carry out this work, we used techniques of dissociation of epidermis and freehand cuts. For dissociation of the epidermis the leaves were sectioned in five regions: apical, median region of the central vein, region close to the central vein, basal and marginal; which were immersed in Jeffrey's mixture for 24 hours, clarified and after washing in water they were stained in astrablau and basic fuchsin. Histological sections were made using a razor blade, clarified in an aqueous bleach solution (1:1), washed in tap water, and stained in astrablau and basic fuchsin. The blade in frontal view shows that the covering tissue is formed by an adaxial epidermis with heterodimensional cells and between them occur trichomes whose basal cell is surrounded by epidermal cells; in the abaxial epidermis the cells are similar to those of the adaxial face, however with a greater number of trichomes, these are simple, tiliform, unicellular, in this we also verify the presence of paracitic type stomas and that this tissue is covered by an ornamented cuticle. The leaf in transversal cut presents sometimes a biseriate palisade parenchyma, occupying 113 of the mesophyll, followed by a lacunose parenchyma, with irregular cells and with intercellular spaces, in these tissues we verified some druse idioblasts, frequent vascular bundles and secretory cells. Many characteristics seen in the specimens studied are common to the Annonaceae family, but A. paludosa also presents characteristics specific to the taxon